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. Author manuscript; available in PMC: 2021 Dec 8.
Published in final edited form as: Biomed Pharmacother. 2021 Mar 23;138:111503. doi: 10.1016/j.biopha.2021.111503

Fig. 4.

Fig. 4.

Glycyrrhizin (GLY) diminishes effects of PMMA particles on the size of osteolytic calvarial lesions in aged mice. (A) Expression of osteoclastogenesis-related Oc-stamp, Dc-stamp, and Acp 5/Trap genes in the dissected calvarial tissues of control (no particle placed) and PMMA particle-induced bone lesions of sham and GLY-treated aged wild type (twenty-four-month-old) mice. μ-CT images and histological evaluation of TRAP osteoclasts (B and C) in the PMMA particle-induced calvarial lesions of aged mice that received: Control (no particles), PMMA particles alone, and PMMA/GLY injection. D: The number of TRAP+ osteoclasts measured in a microscopic field (0.01 mm2) of TRAP-stained sections. Because no significant effect of GLY at 20 mg/kg concentration was observed, only a group of mice treated with 200 mg/kg of GLY solution was evaluated by μ-CT and immunohistochemistry. *p < 0.05, **p < 0.01, ***p < 0.001.