Table 1.
Minimum inhibitory concentrations (MICs) of caspofungin alone and in combination with isavuconazole against Candida auris planktonic cells and one-day-old biofilms
| Planktonic cells | Biofilms | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Median MIC (range) of drug used (50% O.D. reduction in turbidity) | Median MIC (range) of drug used (50% O.D. reduction in metabolic activity) | |||||||||
| Alone | In combination | Alone | In combination | |||||||
| Clades | Isolates | Phenotype | Caspofungin (mg/l) | Isavuconazole (mg/l) | Caspofungin (mg/l) | Isavuconazole (mg/l) | Caspofungin (mg/l) | Isavuconazole (mg/l) | Caspofungin (mg/l) | Isavuconazole (mg/l) |
| South Asian | 10 | Non-aggregative | >1a | >2b (2– >2) | 0.25 (0.25–0.5) | 0.015 (0.008–0.015) | >32c | >2b | 0.5 | 0.015 (0.015–0.06) |
| 12 | Non-aggregative | 1 | 1 (0.5–1) | 0.5 (0.015–0.5) | 0.015 (0.015–0.25) | >32c | >2b | 0.5 | 0.015 (0.008–0.015) | |
| 20 | Non-aggregative | >1a | 2 (2- >2) | 1 | 0.015 (0.015–1) | >32c | >2b | 0.5 | 0.03 | |
| 27 | Non-aggregative | >1a (1- >1) | 1 (0.5– >2) | 0.25 (0.25–0.5) | 0.015 (0.015–0.06) | >32c | >2b | 0.5 | 0.015 (0.008–0.015) | |
| 33 | Non-aggregative | >1a | 0.06 | 0.015 (0.015–0.03) | 0.008 | NA | NA | NA | NA | |
| 82 | Non-aggregative | >1a | 2 | 0.25 (0.25–1) | 0.25 (0.03–0.25) | >32c | >2b | 4 (4–8) | 0.06 (0.008–0.06) | |
| 164 | Non-aggregative | >1a | 0.5 | 0.5 (0.5–1) | 0.06 (0.03–0.06) | NA | NA | NA | NA | |
| 174 | Non-aggregative | >1a | 0.06 | 0.015 (0.015–0.25) | 0.008 | NA | NA | NA | NA | |
| 196 | Non-aggregative | >1a | 0.06 | 0.06 (0.06–0.125) | 0.008 (0.008–0.015) | NA | NA | NA | NA | |
| East Asian | 15 | Aggregative | 0.5 | 0.5 (0.25–0.5) | 0.25 | 0.25 | >32c | >2b | 0.5 | 0.125 |
| 12 372 | Aggregative | 1 | 0.5 | 0.125 | 0.008 (0.008–0.015) | >32c | >2b | 4 | 1 | |
| 12 373 | Aggregative | 0.5 | 0.5 | 0.25 | 0.25 (0.25–0.125) | NA | NA | NA | NA | |
| Type strain (NCPF 13 029) | Non-aggregative | 0.5 | 0.03 | 0.015 | 0.008 | NA | NA | NA | NA | |
| South African | 2 | Aggregative | 1 | 0.125 (0.06–0.125) | 0.015 | 0.008 (0.008–0.015) | NA | NA | NA | NA |
| 185 | Aggregative | 1 | 0.25 (0.125–0.25) | 0.015 | 0.03 (0.03–0.015) | NA | NA | NA | NA | |
| 204 | Aggregative | 0.5 | 0.125 (0.06–0.125) | 0.015 | 0.03 | 32 (16–32) | >2b | 0.5 | 0.008 | |
| 206 | Aggregative | 1 | 0.25 (0.125–0.25) | 0.015 | 0.008 | NA | NA | NA | NA | |
| 228 | Aggregative | 1 | 0.06 | 0.03 (0.015–0.06) | 0.008 (0.008–0.015) | 32 | >2b | 0.5 | 0.008 (0.008–0.015) | |
| South American | I-24 | Non-aggregative | >1a | 1 (0.5–1) | 0.015 (0.015–1) | 0.008 (0.008–0.03) | >32c | 0.5 | 4 | 0.125 |
| I-172 | Non-aggregative | 1 | 0.5 | 0.5 | 0.25 | >32c | 0.5 (0.5–2) | 16 | 0.06 | |
| 13 108 | Non-aggregative | >1a | 2 | 1 | 0.008 | >32c | >2b | >32c | >2b | |
| 13 112 | Non-aggregative | 0.5 | 2 | 0.015 (0.015–0.03) | 0.5 | >32c | >2b | >32c | >2b | |
| 16 565 | Non-aggregative | 0.5 | 0.015 | 0.015 | 0.008 | 2 | 1 (0.5–1) | 0.5 | 0.06 | |
a
MIC is off-scale at >1 mg/l, 2 mg/l (one dilution higher than the highest tested concentration) was used for FICI analysis.
b
MIC is off-scale at >2 mg/l, 4 mg/l (one dilution higher than the highest tested concentration) was used for FICI analysis.
c
MIC is off-scale at >32 mg/l, 64 mg/l (one dilution higher than the highest tested concentration) was used for FICI analysis.