TABLE 1.
Plasmids and strains used in this studya
| Plasmid or strain | Description | Source or reference |
|---|---|---|
| Plasmid | ||
| pKD3 | Template plasmid for RED system, CmR | (52) |
| pKD46 | RED expression plasmid, Ampr | (52) |
| pCP20 | Flp expression plasmid, Ampr | (52) |
| Strain | ||
| WT | Escherichia coli reference strain U9-14, serotype (O2:K1:H4), 3% O-acetylation | This study |
| ΔneuD | Mutant of E. coli U9-14 in which neuD gene was knocked out and lack of K1 capsule | This study |
| NeuO+ | Variant of Escherichia coli U9-14, K1 capsule with 44% O-acetylation | This study |
a
CmR, chloramphenicol resistance; Ampr, antibiotic-resistant bacteria/ampicillin resistance.