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. 2021 Dec 8;9(3):e01803-21. doi: 10.1128/Spectrum.01803-21

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FIG 1 — Sequencing artifact analysis of spike protein amino acid position 142. (A) Median read depths at each nucleotide position for the assembly of the set of 663 V3 (blue) and V4 (red) samples. (B) Median read depth at each primer for the set of 663 V3 assemblies. (C) Median read depth at each primer for the set of 663 V4 assemblies. Orange squares are right primers and blue circles are left primers. (D) The fraction of B.1.617.2 sequences in GISAID with (blue) and without (orange) G142D through August 31, 2021. (E) Frequency of L452R (gray), D950N (blue), and G142D (orange) amino acid substitutions observed in SARS-CoV-2-positive samples from April through August of 2021. L452R, D950N, and G142D are hallmark amino acid substitutions of the Delta variant.