FIG 3.

Overexpression of dccA decreases Spo0A-dependent gene expression. (A and C) qRT-PCR analyses of dccA (A) and sigE (C) transcript levels in R20291 pMC211 (RT526), R20291 pDccA (RT527), and R20291 pDccA^mut (RT539) grown on 70:30 sporulation agar supplemented with 2 μg/ml thiamphenicol and 0 to 0.5 μg/ml nisin, as indicated, at H₁₂. (B and D) qRT-PCR analyses of dccA (B) and sigE (D) transcript levels in 630Δerm pMC211 (RT762) and 630Δerm pDccA (RT763) grown on 70:30 sporulation agar supplemented with 2 μg/ml thiamphenicol in the absence or presence of 0.5 μg/ml nisin at H₁₂. The means and standard errors of the means from at least three biological replicates are shown. *, P < 0.05 (by one-way ANOVA with Dunnett’s posttest comparing values to that of the uninduced WT vector). (E) Anti-Spo0A Western blot analysis of 630Δerm pMC211 (RT762), 630Δerm pDccA (RT763), 630Δerm pDccA^mut (RT764), R20291 pMC211 (RT526), R20291 pDccA (RT527), and R20291 pDccA^mut (RT539) grown on 70:30 sporulation agar supplemented with 2 μg/ml thiamphenicol and 0.5 μg/ml nisin at H₁₂.