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. 2021 Dec 8;6(6):e00919-21. doi: 10.1128/msphere.00919-21

TABLE 1.

Effect of dccA overexpression on sporulation-specific gene expression in R20291 and 630Δerm

Transcripta Strainb Mean fold change ± SDc
pMC211 pDccA pDccAmut
spo0A R20291 0.91 ± 0.12 0.96 ± 0.27 0.77 ± 0.11
630Δerm 0.79 ± 0.07 0.96 ± 0.16 ND
 
murG
(Spo0A dependent)		 R20291 0.85 ± 0.08 0.72 ± 0.08 0.79 ± 0.06
630Δerm 1.19 ± 0.15 0.60 ± 0.05 ND
 
sigF R20291 0.97 ± 0.20 1.15 ± 0.38 0.95 ± 0.23
630Δerm 0.74 ± 0.10 0.47 ± 0.11 ND
 
gpr
(SigF dependent)		 R20291 0.82 ± 0.10 0.6 ± 0.17 0.9 ± 0.10
630Δerm 1.16 ± 0.19 0.44 ± 0.15 ND
 
spoIID
(SigE dependent)		 R20291 0.73 ± 0.14 0.48 ± 0.08 0.67 ± 0.13
630Δerm 1.32 ± 0.13 0.72 ± 0.06 ND
a

All mean fold change values are relative to the respective parent strain containing pMC211 grown in the presence of no nisin.

b

Strains were harvested at H12 from 70:30 sporulation agar supplemented with 2 μg/ml thiamphenicol and 0.5 μg/ml nisin.

c

Boldface type indicates a P value of ≤0.05 by Student’s t test (630Δerm background). There was no statistically significant data in the R20291 background as determined by one-way ANOVA followed by Dunnett’s multiple-comparison test (R20291 background). ND, not determined.