FIG 1.

Schematic overview of the experimental design. A highly saturating Tn5 library was constructed through electroporation of EZ-Tn5 transposome complex to S. Typhimurium 14028s. Approximately 350,000 Tn5 mutants were collected on LB (Km + NA) plates. Complex Tn5 mutant library (IP1) was selected based on growth (LB medium [LB], 100 mM propionate in LB medium [PA], 3% NaCl in LB medium [NaCl], and 1 mM hydrogen peroxide in LB medium [H₂O₂]) and survival (exposed to pH 3 for 30 min [pH 3] and incubated for 12 days in 1× PBS [starvation]). Input pool 2 (IP2) was a technical replicate of input pool 1 (IP1).