FIG 6.

Genotype-phenotype network connections illustrating phenotypic basis of S. Typhimurium genetic factors required for systemic infection (in vitro versus in vivo [systemic]). Large square nodes indicate various conditions (studies), and small nodes are fitness genes. Each node (gene) is shared by at least one of the in vitro conditions (i.e., stressors encountered by Salmonella inside macrophages; NaCl, H₂O₂, pH 3, starvation, and dLB; current study and our previous study) and at least one of the in vivo systemic conditions (MΦ, Sp-Liv, P-Mice, and A-Mice; previous studies). The interactive network through the network data exchange (NDEx) is available at www.ndexbio.org/#/network/5e78ad70-e209-11e8-aaa6-0ac135e8bacf (118).