TABLE 2.

RT-QuIC analysis of endpoint dilutions of input brain and output fecal samples to determine prion recovery

Sample	RT-QuIC results at dilutiona						SD50/gb	Recovered
								Feces (g)	SD50	% SD50
	10−3	10−4	10−5	10−6	10−7	10−8
CWD brain	4/4	4/4	4/4	4/4	2/4	0/4	5 × 109 (2.5 × 1011)
Female, day 3	4/4	4/4	2/4	0/4	NT	NT	5 × 107	195.9	9.8 × 109	3.9
Male, day 3	4/4	3/4	1/4	0/4	NT	NT	1.6 × 107	450.9	7.1 × 109	2.8

^aNumber of positive RT-QuIC reactions over the total number of wells tested. NT, not tested.

^bThe calculated prion seeding activity that would produce positive responses in 50% of replicate RT-QuIC reactions. The value in parentheses is the total SD₅₀ fed to each lion.