KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD3 (17A2) PE-Cy7 | BioLegend | 100220; RRID:AB_1732057 |
| Anti-mouse CD8a (53-6.7) BUV395 | BD Biosciences | 563786; RRID:AB_2732919 |
| Anti-mouse CD8a (53-6.7) BV421 | BioLegend | 100738; RRID:AB_11204079 |
| Anti-mouse CD4 (RM4-5) FITC | BioLegend | 100510; RRID:AB_312713 |
| Anti-mouse CD25 (PC61) APC-Cy7 | BioLegend | 102026; RRID:AB_830745 |
| Anti-mouse CD137 (17B5) APC | BioLegend | 106110; RRID:AB_2564297 |
| Anti-mouse PD-1 (29F.1A12) BV421 | BioLegend | 135218; RRID:AB_2561447 |
| Anti-mouse PD-1 (J43) BV421 | BD Biosciences | 562584; RRID:AB_2737668 |
| Anti-mouse CTLA-4 (UC10-4B9) BV605 | BioLegend | 106323; RRID:AB_2566467 |
| Anti-mouse CD152 (UC10-4F10-11) PE-CF594 | BD Biosciences | 564332; RRID:AB_2732917 |
| Anti-mouse CD161 (PK136) PerCP-Cy5.5 | BioLegend | 108728; RRID:AB_2132705 |
| Anti-mouse CD49b (DX5) PerCP-Cy5.5 | BioLegend | 108916; RRID:AB_2129358 |
| Anti-mouse CD69 (H1.2F3) BV785 | BioLegend | 104543; RRID:AB_2629640 |
| Anti-mouse CD107a (1D4B) BV711 | Biolegend | 121631; RRID:AB_2783065 |
| Anti-mouse CD45.2 (104) BUV737 | BD Biosciences | 612778; RRID:AB_2870107 |
| Anti-mouse Ly-6C (HK1.4) PE-Cy7 | BioLegend | 128018; RRID:AB_1732082 |
| Anti-mouse Ly-6G (1A8) PerCP-Cy5.5 | BioLegend | 127616; RRID:AB_1877271 |
| Anti-mouse CD11c (N418) FITC | BioLegend | 117306; RRID:AB_313775 |
| Anti-mouse CD11b (M1/70) APC-Cy7 | BioLegend | 101226; RRID:AB_830642 |
| Anti-mouse CD24 (M1/69) BV711 | BioLegend | 563450; RRID:AB_2738213 |
| Anti-mouse MHC II (M5/114.15.2) BV605 | BioLegend | 107639; RRID:AB_2565894 |
| Anti-mouse F4/80 (T45-2342) BUV395 | BD Biosciences | 565614; RRID:AB_2739304 |
| Anti-mouse CD86 (GL-1) PE-Dazzle 594 | BioLegend | 105042; RRID:AB_2566409 |
| Anti-mouse CCR7 (4B12) PerCP-Cy5.5 | BioLegend | 120116; RRID:AB_2291144 |
| Anti-mouse CD169 (3D6.112) APC | BioLegend | 142418; RRID:AB_2565641 |
| Anti-mouse CD103 (2E7) PE | BioLegend | 121406; RRID:AB_1133989 |
| Anti-mouse CD206 (C068C2) PerCP-Cy5.5 | BioLegend | 141716; RRID:AB_2561992 |
| Anti-mouse Ki67 (16A8) PE-Dazzle 594 | BioLegend | 652428; RRID:AB_2632696 |
| Anti-mouse CD31 (MEC13.3) AF488 | BioLegend | 102514; RRID:AB_2161031 |
| Anti-mouse CD31 (MEC13.3) AF647 | BioLegend | 102516; RRID:AB_2161029 |
| Anti-mouse CD31 (390) BV605 | BioLegend | 102427; RRID:AB_2563982 |
| Anti-mouse GP38 (8.1.1) APC-Cy7 | BioLegend | 127418; RRID:AB_2629804 |
| Anti-mouse NG-2 (polyclonal) AF488 | Sigma Aldrich | AB5320A4; RRID:AB_11203143 |
| Anti-human/mouse AN2 (NG-2, 1E6.4) PE | Miltenyi Biotec | 130-097-455; RRID:AB_2651235 |
| Anti-mouse VEGFR2 (89B3A5) PerCP-Cy5.5 | BioLegend | 121918; RRID:AB_893646 |
| Anti-mouse PNAd (MECA-79) AF488 | eBioscience | 53-6036-82; RRID:AB_10804391 |
| Anti-mouse CD106 (VCAM-1, 429) AF647 | BioLegend | 105712; RRID:AB_493429 |
| Anti-mouse CD102 (ICAM-1, YN1/1.7.4) BV711 | BioLegend | 116143; RRID:AB_2876429 |
| Anti-mouse CD62E (10E9.6) BV421 | BD Biosciences | 740027; RRID:AB_2739799 |
| Anti-mouse CD45.1 (A20) APC | BioLegend | 110714; RRID:AB_313503 |
| Anti-mouse Thy1.1 (OX-7) BV711 | BioLegend | 202539; RRID:AB_2562645 |
| Anti-mouse IFN-γ (XMG1.2) PE | BioLegend | 505808; RRID:AB_315402 |
| Anti-mouse IFN-γ (XMG1.2) APC | BioLegend | 505810; RRID:AB_315404 |
| Anti-mouse TNF-α (MP6-XT22) FITC | BioLegend | 506304; RRID:AB_315425 |
| Anti-mouse Granzyme B (QA16A02) APC | BioLegend | 372204; RRID:AB_2687028 |
| Anti-mouse CXCL9 (MIG-2F5.5) eFluor660 | eBioscience | 50-3009-80; RRID:AB_11218694 |
| Anti-mouse PD-1 (29F1A12) | BioXCell | BE0273; RRID:AB_2687796 |
| Anti-mouse CTLA-4 (9D9) | BioXCell | BE0131; RRID:AB_10950184 |
| Anti-mouse CD3ε F(ab’)2 (145-2C11) | BioXCell | BE0001-1FAB; RRID:AB_2687679 |
| Anti-mouse CD3ε (145-2C11) | BioXCell | BE0001-1; RRID:AB_1107634 |
| Anti-mouse CD8α (2.43) | BioXCell | BE0061; RRID:AB_1125541 |
| Anti-mouse CD4 (GK1.5) | BioXCell | BE0003-1; RRID:AB_1107636 |
| Anti-mouse NK1.1 (PK136) | BioXCell | BE0036; RRID:AB_1107737 |
| Anti-mouse Gr-1 (RB6-8C5) | BioXCell | BE0075; RRID:AB_10312146 |
| Anti-mouse F4/80 (CI:A3-1) | BioXCell | BE0206; RRID:AB_10949019 |
| Anti-mouse IFN-γ (XT3.11) | BioXCell | BE0055; RRID:AB_1107694 |
| Anti-mouse TNF-α (XMG1.2) | BioXCell | BE0058; RRID:AB_1107764 |
| Anti-mouse CXCL9 (MIG-2F5.5) | BioXCell | BE0309; RRID:AB_2736989 |
| Anti-mouse VEGFR2 (DC101) | BioXCell | BE0060; RRID:AB_1107766 |
| Bacterial and virus strains | ||
| 5-alpha Competent E. coli | New England Biolabs | C2987U |
| Chemicals, peptides and recombinant proteins | ||
| 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] | Layson Bio | 100220 |
| 4-hydroxytamoxifen solution | Sigma Aldrich | SML1666 |
| GolgiPlug Protein Transport Inhibitor (containing Brefeldin A) | BD Biosciences | BDB555029 |
| Cell Stimulation Cocktail | eBioscience | 00-4970-93 |
| FTY720 | Sigma Aldrich | SML0700 |
| Protease Inhibitor Cocktail | Roche | 5892970001 |
| CSVYDFFVWL | Genscript | SC1848 |
| Recombinant murine IFN-γ | Peprotech | 315-05 |
| DNase I | Sigma Aldrich | 10104159001 |
| Collagenase IV | Worthington | LS004188 |
| Collagenase D | Sigma Aldrich | 11088866001 |
| Collagenase P | Sigma Aldrich | 11213865001 |
| Dispase | StemCell Technologies, Inc. | 7913 |
| Critical commercial assays | ||
| ZymoPURE Plasmid Miniprep Kit | Zymo Research | D4210 |
| ExpiFectamine 293 Transfection Kit | Thermo Fisher | A14524 |
| GIBCO ACK Lysing Buffer | Thermo Fisher | A10492-01 |
| CSFE | Thermo Fisher | C34554 |
| CellTrace Violet | Thermo Fisher | C34557 |
| LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation | Thermo Fisher | L34966 |
| FITC Annexin V Apoptosis Detection Kit | BD Biosciences | 556547 |
| Fixation/Permeabilization Solution Kit | BD Biosciences | 554714 |
| Foxp3 / Transcription Factor Staining Buffer Set | eBioscience | 00-5523-00 |
| MACS tissue storage solution | Miltenyi Biotec | 130-100-008 |
| Mouse tumor dissociation kit | Miltenyi Biotec | 130-096-730 |
| Mouse CD45 microbeads | Miltenyi Biotec | 130-052-301 |
| NK Cell Isolation Kit, mouse | Miltenyi Biotec | 130-115-818 |
| Macrophage Isolation Kit (Peritoneum), mouse | Miltenyi Biotec | 130-110-434 |
| EasySep Mouse CD8+ T Cell Isolation Kit | StemCell Technologies, Inc. | 19853 |
| Mouse IFN-γ ELISPOT Kit | BD Biosciences | 551083 |
| VECTASHIELD Antifade Mounting Medium with DAPI | Vector Labs | H-1200-10 |
| Hoechst 33342, Trihydrochloride, Trihydrate, 100mg | Thermo Fisher | H1399 |
| Dextran, Tetramethylrhodamine, 70,000 | Thermo Fisher | D1818 |
| DyLight 649 labeled lectin | Vector Labs | DL-1178 |
| EF5 Hypoxia Detection Kit, Alexa Fluor 488 | Sigma Aldrich | EF5-30A4 |
| Aspartate aminotransferase activity assay kit | Sigma Aldrich | MAK055-1KT |
| Alanine aminotransferase activity assay kit | Sigma Aldrich | MAK052-1KT |
| RNeasy Micro Kit | QIAGEN | 74004 |
| High-Capacity RNA-to-cDNA Kit | Thermo Fisher | 4388950 |
| TaqMan Universal Master Mix | Thermo Fisher | 4440049 |
| Mouse/Rat FLT3L Quantikine ELISA Kit | R&D systems | MFK00 |
| Mouse XCL1/Lymphotactin DuoSet ELISA | R&D systems | DY486 |
| Deposited data | ||
| RNA-seq data | GEO | GEO: GSE184599 |
| Experimental models: Cell lines | ||
| B16F10 cells | ATCC | CRL-6475; RRID:CVCL_0159 |
| TC-1 cells | T. C. Wu Lab at JHU | N/A |
| YUMM1.7 cells | M. W. Bosenberg Lab at Yale | N/A |
| KP cells | T. Jacks Lab at MIT | N/A |
| B16F10-ZsGreen cells | R. Hynes Lab at MIT | N/A |
| Expi293F cells | Thermo Fisher | A14527; RRID:AB_2761403 |
| Experimental models: Organism/strains | ||
| C57BL/6J mice | Jackson Laboratory | 000624; RRID:IMSR_JAX:000624 |
| Batf3−/− mice (B6.129S(C)-Batf3tm1Kmm/J) | Jackson Laboratory | 013755; RRID:IMSR_JAX:013755 |
| Fcgr3−/− mice (B6.129P2-Fcgr3tm1Jsv/2J) | Jackson Laboratory | 009637; RRID:IMSR_JAX:009637 |
| Pmel-1/Thy1.1 mice (B6. Cg-Thy1a/Cy Tg(TcraTcrb)8Rest/J) | Jackson Laboratory | 005023; RRID:IMSR_JAX:005023 |
| IFN-γ reporter mice (B6.129S4-Ifngtm3.1Lky/J) | Jackson Laboratory | 017581; RRID:IMSR_JAX:017581 |
| Ifng−/− mice (B6.129S7-Ifngtm1Ts/J) | Jackson Laboratory | 002287; RRID:IMSR_JAX:002287 |
| BP-SIY mice | Spranger Lab at MIT | N/A |
| Oligonucleotides | ||
| Il10 primer | Thermo Fisher | Mm01288386_m1 |
| Ncr1 primer | Thermo Fisher | Mm01337324_g1 |
| Klra8 primer | Thermo Fisher | Mm01183337_m1 |
| Xcl1 primer | Thermo Fisher | Mm00434772_m1 |
| Flt3l primer | Thermo Fisher | Mm00442801_m1 |
| Tnf primer | Thermo Fisher | Mm00443258_m1 |
| Ifng primer | Thermo Fisher | Mm01168134_m1 |
| Nos2 primer | Thermo Fisher | Mm00440502_m1 |
| H2-Aa primer | Thermo Fisher | Mm00439211_m1 |
| Cxcl9 primer | Thermo Fisher | Mm00434946_m1 |
| Gzmb primer | Thermo Fisher | Mm00442837_m1 |
| Stat1 primer | Thermo Fisher | Mm01257286_m1 |
| Vegfa primer | Thermo Fisher | Mm00437306_m1 |
| Actb primer | Thermo Fisher | Mm02619580_g1 |
| Recombinant DNA | ||
| gWIZ plasmid | Genlantis | P000200 |