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. 2021 Feb 15;236(9):6534–6547. doi: 10.1002/jcp.30328

Figure 1.

Figure 1

Schematic representation of procedures for bioengineering fibrin mouse skeletal muscle (SkM). Macro‐ and microscopic images of C2C12 fibrin bioengineered muscle at (a) 0 days, (b) 3–4 days grown to confluency in 20% heat‐inactivated fetal bovine serum (hiFBS)/heat‐inactivated newborn calf serum (hiNBCS), (c) 5–6 days differentiated in heat‐inactivated horse serum (hiHS) for 48 h and (d) myotubes matured up to 14 days in 7% hiFBS/hiNBCS (10× magnification, scale bar = 50 μm, Olympus, CKX31)