FIGURE 4.

Proteomic profile and protein co-expression network analysis during the fruit development of ‘Junzao’ and ‘Qingjiansuanzao’. (A) Heatmap representation of protein expression profile during fruit development. J20–J100 and S20–S100 represent 20, 40, 60, 80, and 100 DAF of ‘Junzao’ and ‘Qingjiansuanzao,’ respectively. (B) Number of differential expressed proteins (DEPs) detected between neighbor stages and between ‘Junzao’ (indicated by a blue inner circle) and ‘Qingjiansuanzao’ (orange outer circle) at the same stage. (C) Hierarchical cluster tree of co-expression modules identified by weighted gene co-expression network analysis (WGCNA). Each leaf in the tree represents one protein and each tree branch stands for a module. (D) Module–fruit developmental stage associations. The color of each cell at the row–column intersection indicates the correlation coefficient r (up) between the modules (row) and the protein expression profile at a fruit developmental stage (column) and the r-associated significance p-value (down). The WGCNA network of top hub genes in modules Yellow (E), Lightyellow (F), and Black (G). PGDH, D-3-phosphoglycerate dehydrogenase; ACG, glucose-6-phosphate 1-dehydrogenase; SBP, selenium-binding protein 2; SPS, sucrose-phosphate synthase; KAB, voltage-gated potassium channel; PAT, bifunctional aspartate aminotransferase and glutamate; GPX6, phospholipid hydroperoxide glutathione peroxidase 6; LACS2, long chain acyl-CoA synthetase 2; PGIC1, glucose-6-phosphate isomerase; ALDH3H1, aldehyde dehydrogenase family 3 member H1; CCR1, cinnamoyl-CoA reductase 1; APX, ascorbate peroxidase.