TABLE 3.
Transcription signals in plasmid pMV158
| Gene(s) | Promoter(s) | −35 sequencec | Spacer length (nt) | −10 sequencec | Reference |
|---|---|---|---|---|---|
| copG-repB | Pcr | TTGAtt | 17 | TATGCTActAT | 255 |
| tetL | Ptet | TTGACA | 16 | TGTGGTAaAAT | 104 |
| mobM | E. coli: Pmob2 | TgGAag | 17 | TGTGCTAaAcT | 122 |
| L. lactis: Pmob1 | aTGAat | 16 | TGTGtTATAcT | 311 | |
| S. pneumoniae a | Both promoters | Both promoters | 123 | ||
| rnaI | PI | TTGACA | 18 | TGtTATAAT | 117 |
| rnaII | PctII | TTGctt | 17 | TATAAT | 121 |
| ssoA b | PA | TTGACA | 16 | TAgcgT | 147 |
| ssoU b | PU | TaGAaA | 17 | aAattT | 199 |
a
The pneumococcal SigA protein recognized both promoters.
b
On ssDNA.
c
Nonconsensus nucleotides in the sequence are shown in lowercase letters.