TABLE 4.
Efficiency of transfer (ν), copy number (Nav), and replicative indicators (ssDNA/dsDNA) of pMV158 and derivatives in different hosts
| Host/plasmid |
S. pneumoniae
a
|
E. faecalis
a
|
B. subtilis
b
|
E. coli
c
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| νd | N av | ssDNA/dsDNA ratioe | ν | N av | ssDNA/dsDNA ratio | ν | N av | ssDNA/dsDNA ratio | ν | N av | ssDNA/dsDNA ratio | |
| wt | 100 | 34 ± 5 | 1 | 100 | 17 ± 4 | 1 | 100 | 12 ± 4 | 1 | 100 | 8 ± 1 | 1 |
| ΔssoA | 70 | 22 ± 4 | 1 | 100 | 10 ± 3 | 1 | 30 | 8 ± 3 | 1 | 80 | 8 ± 2 | 1 |
| ΔssoU tetL | 70 | 22 ± 2 | 1 | 3 | 5 ± 2 | 20 | 30 | 5 ± 2 | 10 | 80 | 5 ± 2 | 1 |
| ΔssoU cat | 70 | 22 ± 2 | 1 | 3 | 5 ± 2 | 20 | 30 | 5 ± 2 | 10 | 80 | 5 ± 1 | 10 |
| ΔssoA ΔssoU tetL | 20 | 5 ± 1 | 100 | 1 | 3 ± 1 | 50 | 15 | 3 ± 1 | 100 | 0 | —f | — |
| ΔssoA ΔssoU cat | 0 | — | — | NTg | NT | NT | 0 | — | — | 0 | — | — |
a
The method used for transfer was mobilization.
b
The method used for transfer was natural competence.
c
The method used for transfer was electrotransformation.
d
The efficiencies of transfer (ν) were normalized to 100% for the values obtained for pMV158wt in the various hosts.
e
The ssDNA/dsDNA ratio was normalized to 1 for the pMV158wt in the various hosts.
f
—, not measurable.
g
NT, not tested.