TABLE 5.
Plasmid relaxed forms in total DNA extracts and mobilization frequencies in plasmid pMV158 and derivativesa
| Plasmid | Relaxed forms (%) | ν (transconjugants/recipients)b,c |
|---|---|---|
| pMV158 | 77 ± 4 | 102.6 ± 3.5 |
| pMV158mutIR3L | 57 ± 5 | 2.4 ± 0.4 |
| pMV158ΔIR3L | 65 ± 7 | 2.1 ± 0.6 |
| pLS1 (ΔoriT) | <8.3 | <10−10d |
a
The derivatives of pMV158 carry base changes at the left arm of IR3 (mutIR3L), a small deletion at IR3 (ΔIR3L), or a total deletion of the oriT region (pLS1, used as a control).
b
Average result of 4 experiments.
c
ν, mobilization frequency × 10−7.
d
10−10 is the detection limit.