Fig. 4. Live-cell confocal imaging using oxime-caged fluorophores. (A) Synthesis of organelle-targeting BAD-Oxm fluorogenic fluorophores. (B) Photoactivation of BAD-Oxm (5 μM) in A431 cells using 405 nm laser activation. Confocal images were captured before and after 405 nm light activation of BAD-Oxm. Scale bar = 5 μm. (C) Photoactivation of organelle-targeting BAD-Oxm probes (2 μM) in A431 cells. Confocal images were captured from cells incubated with BAD-Oxm-TPP, BAD-Oxm-MOR, and BAD-Oxm-Ts along with corresponding commercial markers for mitochondria, lysosomes, and endoplasmic reticulum, respectively. Commercial MitoView™ 633, LysoView™ 633, and ERTracker™ Red (BODIPY™ TR Glibenclamide) were used as markers for mitochondria, lysosomes, and endoplasmic reticulum, respectively. Scale bar = 5 μm.
