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. 2021 Dec 8;16(12):e0260878. doi: 10.1371/journal.pone.0260878

Fig 1. Glomeruli of PodoPalld129-/- mice have dilated capillaries and show a reduced expression of integrin α8.

Fig 1

(A) The periodic acid staining of paraffin kidney sections revealed dilated capillaries in 6 and 12 months old PodoPalld129-/- mice (asterisks). Scale bar represents 10 μm. (B) Quantitative analysis confirmed that PodoPalld129-/- mice of both age groups have significantly more glomeruli with dilated capillaries than controls of the same age. (C) Immunofluorescence staining of kidney sections showed a marked reduction of integrin α8 expression in PodoPalld129-/- glomeruli. Scale bar represents 20 μm. (D) Western blot quantification of Itga8 showed a significant decrease in 6 months old PodoPalld129-/- glomeruli (KO) compared to the control (WT; 6 months old PodoPalld129+/+ glomeruli protein lysates). Data are presented as means ± SD; * p<0.05; ** p<0.01; **** p<0.001; ns, not significant; two-way ANOVA with FDR correction (B) or unpaired Student’s t-test (D).