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The relative transcriptional level of the two ductal markers EpCAM and SOX9 in each sample condition considered was normalized with the best and the worst reference gene (RPL13A and ACTB, respectively), the geometric mean (Geo.mean) of two selected HKGs by NormFinder (PPIA and UBC) and one commonly used gene (GAPDH). One-way analysis of variance followed by Dunnett’s post-hoc test for multiple comparisons was used.
