Table 2. Gene expression stability rankings of the twelve reference genes calculated by NormFinder, geNorm, BestKeeper, ΔCt and RefFinder.
Sample | Gene | NormFinder | geNorm | BestKeeper | Δes | RefFinder | |
---|---|---|---|---|---|---|---|
Stability value | M value | ST.DEV | ST.DEV | Value | Rank | ||
Human Panctreatic Organoids (Passages from 0 to 10) | ACTB | 0,684 | 0,89 | 0,95 | 1,28 | 11,24 | 12 |
B2M | 0,435 | 0,69 | 0,83 | 0,91 | 6,59 | 8 | |
EF1a | 0,506 | 0,67 | 0,78 | 0,92 | 6,24 | 7 | |
GAPDH | 0,380 | 0,69 | 0,64 | 0,93 | 4,23 | 4 | |
GUSB | 0,334 | 0,63 | 0,80 | 0,78 | 3,46 | 3 | |
HPRT | 0,210 | 0,65 | 0,67 | 0,73 | 1,73 | 2 | |
PPIA | 0,416 | 0,72 | 0,68 | 0,88 | 5,69 | 5 | |
RNA18S | 0,711 | 1,28 | 0,79 | 1,50 | 10,49 | 11 | |
RPL13A | 0,247 | 0,61 | 0,62 | 0,73 | 1,19 | 1 | |
TBP | 0,403 | 0,72 | 0,90 | 0,85 | 6,12 | 6 | |
UBC | 0,515 | 0,73 | 0,67 | 0,98 | 7,21 | 9 | |
YWHAZ | 0,493 | 0,81 | 0,94 | 0,95 | 9,43 | 10 |