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. 2021 Dec 8;17(12):e1009905. doi: 10.1371/journal.pgen.1009905

Fig 4. Expression of OsMADS14, 15, 18 and 34 were decreased in spPLDox lines while increased in spPLD-RNAi/Cas9 lines.

Fig 4

Total RNAs were extracted from SAM (~ 1 cm in length) of five-week-old rice plants (~ 7 days before bolting) and expressions of OsMADSs genes were examined by qPCR analysis. Rice plants were grown under LD condition (14-h light / 10-h dark cycle, 28°C) with 70% humidity. Expression levels of examined genes were normalized to ACTIN1 transcript. Experiments were repeated for three times and data were shown as mean ± SD (n = 3). Statistical analysis was performed by Tukey’s test (*, p <0.05; **, p < 0.01; ***, p < 0.001, compared to ZH11).