Fig 4. Paw withdrawal threshold at the (a) ipsilateral (CFA-inoculated) hind paw and (b) contralateral (non-CFA-inoculated) hind paw in the treatment groups on day 0, 7, 15 and 20 (n = 6).

The values are presented as mean ± standard error of the mean (SEM). *p < 0.05 Statistical comparison to control group and ^#p < 0.05 Statistical comparison to CFA 7.5. CFA 10.0 group showed a consistent significant reduction in paw withdrawal threshold compared to the control group, indicating the development of tactile allodynia in the rat, beginning with the ipsilateral followed by contralateral hind paws.