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. Author manuscript; available in PMC: 2021 Dec 8.
Published in final edited form as: Nat Aging. 2021 Jul 12;1:598–615. doi: 10.1038/s43587-021-00082-y

Fig. 7 |. CXCL9 regulates endothelial cell senescence and capillary network formation in vivo.

Fig. 7 |

a, Growth curves over 4 d show recovery of cell proliferation in CXCL9-KD iPSC-ECs in later passages when compared to Scramble iPSC-ECs (Padj value of P8 Scramble (day 4) versus P8 CXCL9-KD (day 4) = 0.0232). b, Cellular senescence activity assay shows restoration of SA-β-gal activity in CXCL9-KD iPSC-ECs at later passages when compared to Scramble iPSC-ECs (Padj value of P6 (Scramble) versus P6 (CXCL9 shRNA) = 0.0406; Padj value of P8 (Scramble) versus P8 (CXCL9 shRNA) = 0.0278). c, Representative immunohistochemical images showing CD31+ human capillaries from serially passaged Scramble and CXCL9-KD iPSC-ECs. Arrows denote CD31 staining on iPSC-EC indicating capillary formation. d, Quantification of CD31+ capillaries show improved capacity of late passaged CXCL9-KD iPSC-ECs to form in vivo capillary networks (Padj value of P0 (Scramble) versus P8 (Scramble) <0.0001; Padj value of P8 (Scramble) versus P8 (CXCL9 shRNA) = 0.0487). All data are represented as mean ± s.e.m., n = 3, *P < 0.05, ****P < 0.001. Statistical analyses were performed using one-way ANOVA corrected with the Bonferroni method. Scale bars, 100 μm.