Skip to main content
. 2021 Dec 8;7(50):eabl4024. doi: 10.1126/sciadv.abl4024

Fig. 1. Obesity- and cold temperature–induced changes in vascular function is closely related to alterations in UCP1 expression in PVAT.

Fig. 1.

(A to F) Six-week-old male C57BL/6J mice were fed with standard chow (STC) or high-fat diet (HFD) for 12 weeks before aorta and PVAT were isolated for analysis. (A) Immunohistochemical staining of UCP1 in PVAT. Scale bars, 100 μm. (B) Western blot analysis of UCP1 in PVAT. (C) Pulse wave velocity in thoracic aorta of the mice was measured by ultrasonography. n = 6, *P < 0.05 versus STC mice. (D) Real-time polymerase chain reaction (PCR) analysis for proinflammatory genes in mouse aorta, normalized to rps18, n = 6, *P < 0.05 versus STC mice. (E and F) Acetylcholine (ACh)–induced vasorelaxation in aortic rings without (E) or with (F) PVAT, assessed using wire myographs. n = 7 to 8, *P < 0.05 versus STC mice. (G to J), C57BL/6J mice on HFD were housed at cold (6°C) or ambient temperature (24°C) for 6 days before analysis. (G) Immunohistochemical staining of UCP1 in PVAT. Scale bars, 100 μm. (H) Western blot of UCP1 in PVAT. (I) ACh-induced vasorelaxation in aortic rings with or without PVAT. n = 13 to 15, *P < 0.05 versus 24°C. (J) The mRNA expression of proinflammatory genes in mouse aorta. n = 6, *P < 0.05 versus 24°C.