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. 2021 Dec 8;7(50):eabj1598. doi: 10.1126/sciadv.abj1598

Fig. 6. Hopx-derived NEPs in the BgL turn on ASCL1 upon injury.

Fig. 6.

(A to M) IF analysis (A to F) and quantification (M) of the percentage of the TdT+ and ASCL1+ cells per TdT+ cell in the BgL at P7 on sections from Hopx-TdT nonIR (A, C, and E) and IR (B, D, F, and G to L) brains (n = 3 brains, Student’s t test, P = 0.004). (G) to (L) are taken from the region indicated in (B) and show orthogonal views demonstrating colocalization of TdT+ and ASCL1+ in the BgL in IR Hopx-TdT cerebella. (N) Quantification of the percentage of Hopx-derived TdT+ cells in all BgL-NEPs and Bg (SOX2+ cells) or in all Ascl1+ BgL-NEPs (n = 3 per condition, two-way ANOVA, F(1,8) = 7.928, P = 0.02). Scale bar, 50 μm.