Skip to main content
. 2021 Nov 30;10:e74758. doi: 10.7554/eLife.74758

Figure 3. Parallel output populations in ventral CA1/subiculum.

Figure 3.

(A) Schematic of experiment, three differently tagged CTXβ tracers were injected into prefrontal cortex (PFC), nucleus accumbens (NAc) and basal amygdala (BA). (B) Example injection sites in each region. Scale bar 1 mm. (C) Horizontal section of CA1/subiculum in ventral hippocampus (vH) showing interspersed but non-overlapping labelling. Scale bars 300 µm (left), 100 µm (right). (D) Proportion of neurons labelled with CTXβ injection in NAc (red), BA (green) or PFC (grey) co-labelled with CTXβ from a different region. Note that there is only a small proportion of dual labelled neurons. (E) Strategy for electrophysiology recordings – projection populations were fluorescently labelled with retrobead injections into downstream projection areas. (F) Examples of positive (+160 pA) and negative (–40 pA) current steps in fluorescently targeted neurons from each population. Scale bar = 30 mV, 100 ms. (G, H) No large differences in input/output curve, resting potential, input resistance or sag amplitude across the three populations.

Figure 3—source data 1. Source data for Figure 3.