Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Mus musculus) |
Slc32a1(VGAT)- IRES-Cre(vGAT-cre) |
Jackson Laboratory | Stock #016962;RRID:IMSR_JAX:016962 | |
| Genetic reagent (M. musculus) |
Ai14(RCL- tdT)-D(reporter mice) |
Jackson Laboratory | Stock #007914;RRID:IMSR_JAX: 007914 | |
| Genetic reagent (virus) |
AAV2/1- CaMKII- GFP |
Addgene | Stock #64545-AAV1 | A gift from Edward Boyden |
| Genetic reagent (virus) |
AAV2retro- CAG-Cre |
UNC vector core (Tervo et al., 2016) | ||
| Genetic reagent (virus) |
AAV2/1-EF1a- FLEX-hChR2 (H134R)- EYFP |
Addgene | Stock #20298-AAV1 | A gift from Karl Deisseroth |
| Genetic reagent (virus) |
AAV2/1-hSyn- hChR2 (H134R)-EYFP |
Addgene | Stock #26973-AAV1 | A gift from Karl Deisseroth |
| Genetic reagent (virus) |
AAV2/1-CaMKII-hChR2 (H134R)-EYFP |
Addgene | Stock #26969-AAV1 | A gift from Karl Deisseroth |
| Genetic reagent (virus) |
pAAV2/8-hSyn-dF-HA-KORD- IRES-mCitrine |
Addgene | Stock #6541-AAV8 |
A gift from Bryan Roth |
| Genetic reagent (virus) |
AAV2/1.CAG. FLEX. Ruby2sm- Flag.WPRE |
Addgene | Stock #98928-AAV1 | A gift from Loren Looger |
| Genetic reagent (virus) |
AAV2/9-mDlx- NLS-mRuby2 |
Addgene | Stock #99130-AAV1 | A gift from Viviana Gradinaru |
| Genetic reagent (virus) |
pAAV2/1-Ef1a- DIO mCherry |
Addgene | Stock #114471-AAV1 | A gift from Karl Deisseroth |
| Antibody | Anti-somatostatin antibody, clone YC7 (monoclonal) |
Merck Millipore | MAB354;RRID:AB_2255365 | IHC (1:500) |
| Chemical compound, drug |
Salvinorin B (SalB) |
Hello Bio | HB4887 | |
| Chemical compound, drug |
Cholera Toxin Subunit B (recombinant), Alexa Fluor 647 Conjugate |
Thermo Fisher Scientific | C34778 | |
| Chemical compound, drug |
Cholera Toxin Subunit B (recombinant), Alexa Fluor 594 Conjugate |
Thermo Fisher Scientific | C34777 | |
| Chemical compound, drug |
Cholera Toxin Subunit B (recombinant), Alexa Fluor 488 Conjugate |
Thermo Fisher Scientific |
C34775 | |
| Software, algorithm |
Python 3.7 | https://www.python.org/ | RRID:SCR_008394 | |
| Software, algorithm |
Jupyter Notebook |
https://www.jupyter.org/ | RRID:SCR_018315 | |
| Software, algorithm |
ImageJ (Fiji) | https://www.fiji.sc/ | RRID:SCR_002285 |