Figure 5.

Elevation of the intracellular lipid hydroperoxide tone with T-butyl-OH does not antagonize the inhibitory effect of paracetamol on the diclofenac-induced cyclooxygenase-2 activity at 48 h in J774.2 macrophages. For the experimental protocol, refer to the methods section. ***P < 0.001 diclofenac-stimulated cells vs. unstimulated cells; #P < 0.05 and ##P < 0.01 diclofenac + paracetamol treatment ± T-butyl-OH vs. diclofenac-stimulated cells (ANOVA and Dunnett’s post hoc test). Inset: ***P < 0.001 cells stimulated with diclofenac ± 10, 100, 1000 µM paracetamol vs. unstimulated cells, # P < 0.05 cells stimulated with diclofenac and treated with T-butyl-OH and 10, 100 or 1000 µM paracetamol vs. cells stimulated with diclofenac. Reproduced from Ayoub et al. 2011 [72]