FIG. 2.

Expression analysis of EID-1. (A) Blots containing poly(A)⁺ RNA from multiple samples of adult human tissue were hybridized with the radiolabeled 350-bp human EID-1 cDNA. A single 1.7-kb transcript was detected. High-level expression was detected in cardiac and skeletal muscle tissue. (B) Total RNA obtained from murine ventricles at the indicated developmental time points or adult murine atrial tissue was probed with a corresponding murine EID-1 probe. (C) Protein extracts from primary human skeletal myotubes, differentiated for the indicated times, were probed with polyclonal anti-EID-1 antibody (GN735) and antibodies to the indicated proteins.