FIG. 7.

The C-terminal and acidic domains mediate inhibition of MyoD-dependent transcription by EID-1. To determine the domain or domains within EID-1 responsible for its inhibitory effects, we created multiple-deletion mutations spanning the coding sequences of EID-1. U2OS cells were transfected with 1 μg each of MyoD and 4Rtk-Luc and indicated mutations as described below. Luciferase activity, corrected for that of LacZ, is shown relative to expression in cells receiving MyoD in the absence of EID-1. Amount of EID-1 vectors, 1 μg (A and C) and 0.5, 1, or 2 μg (B and D).