TABLE 2.

Complementation of the P86 mutation by HSP104^a

[URE3-1] donor	Recipient P86 strain	Plasmid	Growth on USA	Feeding on USAb by:
				First cytoductants	Second cytoductants
4645-8CU	4706-1CρO	CEN vector	−	0/12
4645-8CU		2μm vector	−	0/12
4645-8CU		CEN HSP104	+	12/12	2/2
4645-8CU		2μm HSP104	+	12/12	2/2
3310	4706-1DρO	CEN vector	−	0/12
3310		2μm vector	−	0/12
3310		CEN HSP104	+	12/12	14/24
3310		2μm HSP104	+	12/12	24/24

^aP86 strains 4706-1Cρ^O and 4706-1Dρ^O were transformed with the indicated TRP1 and HIS3 plasmids, respectively, and the transformants were used as cytoduction recipients from the [URE3] strains 3310 and 4645-8CU. These first cytoductants were tested for growth and feeding on USA and then used as donors in a second cytoduction to wild type [ure-o] recipients (strains 3310S and 4645-8C). The resulting second cytoductants were then tested for feeding on USA. Plasmids used were pH218 (CEN HIS3 HSP104), pH219 (CEN TRP1 HSP104), pRS423 (2μm HIS3), pRS424 (2μm TRP1), pRS313 (CEN HIS3), pRS314 (CEN TRP1), pH220 (2 μm HIS3 HSP104), and pH221 (2μm TRP1 HSP104). 

^bValues are numbers of colonies showing feeding/total number of colonies tested.