TABLE 3.
Complementation of null mutants (hsp104::G418) by HSP104-carrying plasmidsa
| Plasmid | Growth on USA of cytoductants | Uracil feeding of cytoductantsb |
|---|---|---|
| None | − | |
| pRS313 (CEN vector) | − | 0/24 |
| pRS423 (2μm vector) | − | 0/24 |
| pH218 (CEN HSP104) | + | 24/24 |
| pH220 (2μm HSP104) | + | 24/24 |
a
The hsp104::G418 strain 4751-11A was transformed with the indicated plasmids, and [URE3-1] was introduced into the transformant by cytoduction from [URE3-1] donor strain 4645-8CU.
b
Numbers of colonies showing feeding/total number of colonies tested.