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High-copy-number HSP104 plasmids do not cure [URE3]a
| Plasmid | No. of colonies positive for [URE3]/total no. tested
|
|
|---|---|---|
| Vector | HSP104 | |
| CEN TRP1 | 50/52 | 41/49 |
| CEN HIS3 | 49/55 | 40/44 |
| 2μm TRP1 | 40/42 | 43/45 |
| 2μm HIS3 | 41/44 | 41/41 |
Transformants of YHE835 with the CEN and 2μm plasmids with and without HSP104 driven by its own promoter were tested for [URE3] by the feeding test.
