Table 2.
Group comparisons on video-EEG monitoring, motor-type seizures and SWD bursts
| Feature | DLP | DLP-RAP633 | Sham | Control |
|---|---|---|---|---|
| Rats / group | 12 | 10 | 8 | 11 |
| Age at onset of monitoring (PN day) | 78.8 ± 8.8 | 69.9 ± 6.8 | 122.4±37.5 | 93.4±36.8 |
| Age at end of monitoring (PN day) | 177.9 ± 79.4 | 169.5±69.3 | 239.8±50.2 | 247.4±32.7 |
| Rat-days of monitoring | 35.9±25.2 | 46.6±26.7 | 23±11.2 | 65.9±35.3 |
| Motor-type seizures | ||||
| Rats with seizures | 8/12 (66.7%) | 2/10 (20%) | 0/10 (0%) | 1/11 (9.1%) |
| Total seizures (n) | 26 total; 25 with EEG |
12 | 0 | 1 |
| Mean age at first detection (PN day) | 110.3 ± 30.3 (range 68–137) |
140.5 ± 64.3 (range 95–186) |
N/A | 173 |
| Incidence of seizures | 66.7% (8/12 rats) |
20% (2/10 rats) |
0% (0/8) |
9.1% (1/11) |
| State dependence | n of seizures (%) | n of seizures (%) | n of seizures (%) | |
| SWS | 20/25 (80%) | 8/12 (66.7%) | N/A | N/A (no pre-ictal) |
| AW | 5/25 (20%)# | 4/12(33.3%) | N/A | N/A (no pre-ictal) |
| Mean electrographic seizure duration (sec) (Min-max) | 83 ± 101 (range 17 – 481 sec) |
157 ± 112 (range 62–360 sec) |
N/A | End of seizure only |
| Stage 1 * | 1/18 (5.6%) | 0/12 (0%) | N/A | 0/1 (0%) |
| Stage 2 | 1/18 (5.6%) | 0/12 (0%) | N/A | 1/1 (100%) |
| Stage 3 | 7/18 (38.9%) | 1/12 (8.3%) | N/A | 0/1 (0%) |
| Stage 4 | 3/18 (16.7%) | 1/12 (8.3%) | N/A | 0/1 (0%) |
| Stage 5 | 1/18 (5.6%) | 0/12 (0%) | N/A | 0/1 (0%) |
| Stage 6 | 4/18 (22.2%) | 8/12 (66.7%) | N/A | 0/1 (0%) |
| Stage 7 | 0/18 (0%) | 2/12 (16.7%) | N/A | 0/1 (0%) |
| Stage 8 | 1/18 (5.6%) | 0 (0%) | N/A | 0/1 (0%) |
| NCSE | 1/18 (5.6%) | 0 (0%) | N/A | 0/1 (0%) |
| No video | 8/26 (30.8%) | 0 (0%) | N/A | 0/1 (0%) |
| First change in: | ||||
| EEG | 10/17 (58.8%) | 12/12 (100%) | N/A | N/A |
| Behavior | 2/17 (11.8%) | 0/12 (0%) | N/A | N/A |
| Both | 5/17 (29.4%) | 0/12 (0%) | N/A | N/A |
| Unclear/no video | 8/26 (30.8%) | 0 | N/A | 0/1 (0%) |
| Unclear/no EEG | 1/26 (3.8%) | 0 | N/A | 1/1 (100%) |
| SWD bursts | ||||
| Rats with SWD bursts | 4/12 (33.3%) | 5/10 (50%) | 5/8 (62.5%) | 7/11 (63.6%) |
| Mean age at first detection (PN day) | 79 ± 7.5 | 107 ± 57.1 | 144.8 ± 22.8 | 146.6 ± 24.4 |
| Main SWD frequency (Hz) | 5.4 ± 0.8 | 5.8 ± 1.1 | 8.3 ± 0.3 | 7.6 ± 0.5 |
AW: awake; N/A: Not applicable; NCSE: nonconvulsive status epilepticus; PN day: postnatal day; SWS: slow wave sleep.
#:
Seizures in AW state occurred in two rats with NCSE or very epileptic background.
*:
Seizure semiology in this stage 1 seizure was also described as being “jerky, ataxic, restless”