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. 2021 Nov 20;23:560–570. doi: 10.1016/j.omto.2021.11.010

Figure 1.

Figure 1

Knockdown of SOS1 markedly inhibited the cell viability of CML cells in vitro and in vivo

(A) qPCR assays examined SOS1 mRNA levels in cells relative to GAPDH mRNA. (B) Top: expression screening was tested in protein level using western blotting. Bottom: in CML patients, expression of SOS1 can also be higher than in normal subjects. (C and D) Downregulation of SOS1 by transfection with SOS1-siRNA#3 significantly inhibited the cell viability of K562 cells, and the result was consistent with KCL-22 cells. (E) Downregulation of SOS1 by transfecting SOS1-siRNA markedly inhibited cell colony formation ability in K562 cells. (F) In contrast, SOS1 overexpression increased cell colony formation ability. (G) The tumor volume of the K562-SOS1-siRNA#3 group was significantly smaller than that of the K562-NC (negative control)-siRNA and K562-BK (blank) groups. Tumor weight in the two groups was recorded (n = 4 per group). Significance was determined using Student’s test comparing SOS1-siRNA#3 with NC-siRNA (p = 0.02 for volume, p = 0.03 for weight).