Table-1.
The list of primers with thermal conditions used in this study.
| Primer | Sequence(5’-3’) | Target | Amplicon size | PCR condition (35 cycles) | Reference | ||
|---|---|---|---|---|---|---|---|
|
| |||||||
| Denature | Annealing | Extension | |||||
| Cpa-F | GTTGATAGCGCAGGACATGTTAAG | cpa (Alpha toxin) | 402 bp | 94°C, 30 s | 59°C, 45 s | 72°C, 45 s | [14] |
| Cpa-R | CATGTAGTCATCTGTTCCAGCATC | ||||||
| Cpb-F | ACTATACAGACAGATCATTCAACC | cpb (Beta toxin) | 236 bp | 94°C, 30 s | 59°C, 45 s | 72°C, 45 s | |
| Cpb-R | TTAGGAGCAGTTAGAACTACAGAC | ||||||
| Etx-F | ACTGCAACTACTACTCATACTGTG | Etx (Epsilon toxin) | 541 bp | 94°C, 30 s | 59°C, 45 s | 72°C, 45 s | |
| Etx-R | CTGGTGCCTTAATAGAAAGACTCC | ||||||
| Cpi-F | GCGATGAAAAGCCTACACCACTAC | cpi (Iota toxin) | 317 bp | 94°C, 30 s | 59°C, 45 s | 72°C, 45 s | |
| Cpi-R | GGTATATCCTCCACGCATATAGTC | ||||||
PCR=Polymerase chain reaction