Decreased levels of METTL3 and ALKBH5 alters cellular and EV small RNA levels. A) Western blots were performed on cell lysates from stable lines expressing an empty shRNA vector (Empty), a scrambled control shRNA vector, or two independent shRNAs targeting METTL3 or ALKBH5. Representative immunoblots using antibodies against METTL3, ALKBH5, Tubulin, and GAPDH are shown with the average decrease in METTL3 and ALKBH5 being 60.4 ± 1.1% and 68.4 ± 1.04%, respectively. B) qRT/PCR analysis of miR-100 levels in cellular (black) and EV (gray) samples. Significance was determined by one-way ANOVA. Data represent mean ± SE, n = 3, and ∗p < 0.05.