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. 2021 Dec 2;7(12):e08519. doi: 10.1016/j.heliyon.2021.e08519

Figure 3.

Figure 3

Knockdown of METTL3 reduces EV-mediated anchorage-independent growth. A) Gene ontology analysis of significant biological processes affected in METTL3 knockdown cells compared to DKO-1 control cells after total RNAseq. B) Immunoblots of EVs from DKO-1 or METTL3 knockdown cells using antibodies against CD63, TSG101, CD81, and H3. C,D) DKO-1 and METTL3 knockdown cells (500 cells/well) were grown in soft agar for 2 weeks in the presence or absence of EVs derived from either DKO-1 cells or METTL3 knockdown cells. Representative images (C) and quantification of colony counts (D) are shown. Significance was determined by one-way ANOVA. Equivalent amounts of EVs were added. Data represent mean ± SE, n = 3, ∗p < 0.05.