Table 1.

Downregulated miRNAs contain m⁶A consensus sequences.

Cells
miRNA	Read Base Mean	Sequence	FC	P-value	Adjusted P-value
miR-125b-5p	360.877	UCCCUGAGACCCUAACUUGUGA	-1.7	5.07E-09	2.91E-06
miR-100-5p	1252.25	AACCCGUAGAUCCGAACUUGUG	-1.7	3.7E-08	1.06E-05
let-7e-5p	410.781	UGAGGUAGGAGGUUGUAUAGUUgaggaggaca	-1.3	1.91E-06	0.00036
miR-4521	114.646	GCUAAGGAAGUCCUGUGCUCAGuuuuguagcaucaaaacu	-1.0	6.32E-05	0.008
miR-142-5p	121.025	CAUAAAGUAGAAAGCACUACU	-1.2	7.13E-05	0.008
miR-146a-5p	149.772	UGAGAACUGAAUUCCAUGGGUU	-1.0	9.86E-05	0.009
miR-99b-5p	844.702	CACCCGUAGAACCGACCUUGCG	-1.2	0.00017868	0.015
miR-125a-5p	1040.74	UCCCUGAGACCCUUUAACCUGUGA	-1.0	0.00045275	0.026
miR-135b-5p	127.410	UAUGGCUUUUCAUUCCUAUGUGAuugcugucccaaacu	-1.1	0.00238943	0.099
miR-32-5p	241.298	UAUUGCACAUUACUAAGUUGCA	-1.0	0.0222186	0.308
EVs
miRNA	Expression Level	Sequence	FC	P-value	Adjusted P-value
miR-100-5p	1252.25	AACCCGUAGAUCCGAACUUGUG	-1.5	1.09E-06	0.00074
miR-99b-5p	844.702	CACCCGUAGAACCGACCUUGCG	-1.4	1.07E-05	0.003
miR-193b-3p	582.185	AACUGGCCCUCAAAGUCCCGCU	-1.0	1.39E-05	0.003
miR-125b-5p	360.877	UCCCUGAGACCCUAACUUGUGA	-1.2	7.31E-05	0.001
let-7e-5p	410.781	UGAGGUAGGAGGUUGUAUAGUUgaggaggaca	-1.1	0.00014876	0.011
miR-125a-5p	1040.74	UCCCUGAGACCCUUUAACCUGUGA	-1.2	0.00020023	0.013
miR-224-5p	1469.11	UCAAGUCACUAGUGGUUCCGUUUAG	-1.0	0.00274813	0.098

Downregulated miRNAs in METTL3 knockdown cells and EVs compared to DKO-1 cells and EVs. Expression levels (Read Base Mean) are the average number of RNAseq reads from 3 independent libraries. The sequence of downregulated miRNAs is shown along with the log2 fold change (FC), p-values, and adjusted p-values when comparing cell and EV levels between METTL3 knockdown cells and control DKO-1 cells after DESeq2 normalization. Highlighted in red are the m⁶A consensus sequences present in the mature miRNA sequence (upper case) or the immediately adjacent loop sequence in the pre-miRNA.