. 2021 Dec 8;11:23575. doi: 10.1038/s41598-021-03032-1

Table 2.

In-vitro callus induction and callus growth after 30 days of culturing of S. costus using seed, leaf, petiole and root as an explants.

Treatments	Conc. 2,4-D/Kinetin mgL^-1	Type of explants used	Means days to callus induction (x- ± SE)	Callus growth after 30 days (x- ± SE)
T1	0.25/0.5	Seeds	14.00 ± 1.09^A	0.22 ± 0.07^D
		Leaf	15.20 ± 0.73^C	1.50 ± 0.08^AB
		Petiole	15.80 ± 0.73⁴^BC	0.91 ± 0.02^D
		Root	16.40 ± 0.97^A	0.91 ± 0.05B^C
T2	0.5/0.5	Seeds	17.60 ± 0.67^A	1.39 ± 0.06^CD
		Leaf	17.20 ± 0.37^C	1.34 ± 0.04^AB
		Petiole	17.80 ± 0.37^AB	1.27 ± 0.08^BC
		Root	19.20 ± 0.37^AB	0.92 ± 0.02^B
T3	0.5/1.0	Seeds	13.40 ± 0.60^A	1.86 ± 0.06^A
		Leaf	15.80 ± 1.07^C	1.65 ± 0.06^A
		Petiole	15.60 ± 0.50^C	1.42 ± 0.05^A
		Root	17.40 ± 0.86^BC	1.114 ± 0.07^A
T4	0.5/1.5	Seeds	16.60 ± 0.60^A	1.62 ± 0.05^B
		Leaf	18.60 ± 1.07^AB	1.32 ± 0.08^AB
		Petiole	18.60 ± 0.50^A	1.33 ± 0.02^AB
		Root	17.20 ± 0.86^BC	0.75 ± 0.08^C
T5	0.5/2.0	Seeds	17.40 ± 1.07^A	1.54 ± 0.08^BC
		Leaf	18.20 ± 0.66^AB	1.23 ± 0.06^BC
		Petiole	18.40 ± 0.50^A	1.26 ± 0.03^BC
		Root	19.40 ± 0.74^AB	0.90 ± 0.01^BC
T6	1.0/2.0	Seeds	18.20 ± 0.80^A	1.65 ± 0.06^B
		Leaf	19.60 ± 0.81^A	0.92 ± 0.20^C
		Petiole	19.01 ± 0.89^A	1.18 ± 0.06^C
		Root	20.80 ± 0.73^A	0.84 ± 0.03^BC