Fig. 5. The effects of running exercise and fluoxetine on the OPC proliferation and differentiation in the DG subfield of the hippocampus.

a Colocalization of PDGFα⁺ and Olig2⁺ cells in the DG hippocampal subfield. PDGFα⁺: red, Olig2⁺: green, DAPI: blue. The white arrows indicate the PDGFα⁺/Olig2⁺/DAPI co-labeled cells. Scale bar = 50 μm. b The number of PDGFα⁺/Olig2⁺ OPCs in the DG subfield of mice in the CTRL group, UCS group, UCS + RN group, and UCS + FLX group (n = 5 for each group). c Immunofluorescence analyses of the PDGFα⁺/Olig2⁺ cells in the hilus and molecular layer of mice in the CTRL group, UCS group, UCS + RN group, and UCS + FLX group (n = 5 for each group). d Colocalization of the CC1⁺, Olig2⁺, and BrdU⁺ cells in the DG subfield of the hippocampus. CC1⁺: red, Olig2⁺: green, BrdU⁺: blue. The white arrows indicate the CC1⁺/Olig2⁺/BrdU⁺ co-labeled cells, and the pink arrows indicate the Olig2⁺/BrdU⁺ co-labeled cells. Scale bar = 50 μm. e–f Immunofluorescence analyses of the BrdU⁺/Olig2⁺ and CC1⁺/BrdU⁺/Olig2⁺ cells in the DG subfield of mice in the CTRL group, UCS group, UCS + RN group, and UCS + FLX group (n = 5 for each group). All data are shown as the means ± SDs. * P < 0.05 and **P < 0.01.