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. 2021 Dec 8;11:23635. doi: 10.1038/s41598-021-02624-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Feces from park²⁵ flies reduces total pupation rate. Total pupation rates were calculated in control, heterozygous (Het) park²⁵ and homozygous (Hom) park²⁵ flies from the 60 embryos that were placed on food that had control or park²⁵ feces present. Data are presented as mean and SEM. Asterisks represent the results of a post-hoc Sidak’s multiple comparisons test comparing the two feces groups within each fly genotype (**** = P < 0.0001). Results are from 45 separate vials in each group.