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. 2021 Dec 8;7:380. doi: 10.1038/s41420-021-00773-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Representative immunostaining images of Claudin-1/ZO-1 (A), Claudin-5/ZO-1 (B) and quantification of Claudin-1 (C), Claudin-5 (D), and ZO-1 (E) of BMECs derived from CRTC1 KO and WT, before and after ischemia/reperfusion (Scale Bar, 100 μm). F qPCR results of Claudin-1 in BMECs derived from CRTC1 KO and WT mice, before and after ischemia/reperfusion. n = 4 for each group.