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. 2021 Dec 8;11:23607. doi: 10.1038/s41598-021-03013-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype and cytokine profile of each fraction of DP T cells, and differences in expression of CD8αα⁺, CD8αβ⁺, and CD31 by the naïve population. (a) Comparison of cell surface marker and cytokine expression among the CD4^hiCD8^lo, CD4^hiCD8^hi, and CD4^loCD8^hi fractions. (b) Percentage of CD8αα⁺ and CD8αβ⁺ cells within the naïve (CD28⁺CD95⁻) CD8 SP T and DP T cell populations. (c) Percentage of CD4, CD8, and DP T cells expressing CD31, and comparison of percentage of CD8αα⁺CD31⁺ and CD8αβ⁺CD31⁺ cells within the CD8 SP T and DP T cell populations. Data are expressed as the mean ± SDs (n = 4). Dot plots are representative of experimental data, all with similar results. Red dots on the bar graph represent data from the monkey used for scRNA-seq analysis. *p < 0.05; **p < 0.01. ns not significant, GZMB granzyme B.