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. 2021 Dec 8;11:23607. doi: 10.1038/s41598-021-03013-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotype and cytokine profile of the CD4^loCD8^hi fraction. (a) Percentage of NKG2D⁺, perforin⁺, and CD107a⁺ cells within each DP T cell fraction. (b) Percentage of EOMES⁺ cells within each DP T cell fraction. (c) Comparison of granzyme B⁺ and perforin⁺ cells between NKG2D⁺ and NKG2D^- DP T cell fractions. Cytokine production was measured after stimulation of PBMCs with PMA/ionomycin for 4 h. Data are expressed as the mean ± SDs (n = 4). Red dots on the bar graph represent data from the monkey used for scRNA-seq analysis. *p < 0.05; **p < 0.01; ns not significant, GZMB granzyme B.