Skip to main content
. 2021 Dec 8;12:7115. doi: 10.1038/s41467-021-27426-x

Fig. 5. Relative expression of cytokine mRNAs by LA-Mφ with depolarized mitochondria.

Fig. 5

a, b Monocytes were polarized into macrophages by incubation in the absence (Mφ) or presence of lactic acid (LA-Mφ) for 3 days. LA-Mφ with and without depolarized mitochondrial membranes (“Depol” and “Pol” populations, respectively) were sorted by flow cytometry with MitoTracker probes. Cells were unstimulated (a) or stimulated with LPS for 3 h (b) and the levels of TNFα and IL-6 mRNA were assessed by RT-qPCR. The results were expressed as mRNA levels relative to those for the housekeeping gene RPS18 (mean ± SD, a n = 4; b n = 5 for Mφ and LA-Mφ; n = 7 for “Depol” and “Pol” populations). A two-tailed Mann–Whitney U test was performed for statistical analysis. Source data are provided in a Source Data file.