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. 2021 Dec 8;11:23657. doi: 10.1038/s41598-021-02970-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Percent changes (%) in dry matter intake (DMI) per mBW (a), energy balance (EB) (b) and energy corrected milk yield (ECM) (c) after i.p. injections with NaCl (n = 7), AEA (n = 7) or 2-AG (n = 6) under free-ranging, non-stressed conditions in the barn (TB), and under stressed conditions in the respiration chamber (TC) relative to pre-treatment. Change of housing to TC significantly decreased DMI/mBW, EB and ECM in all treatment groups (P < 0.05). Significant pairwise effects within-housing were detected only under stressed conditions in the respiration chamber (TC). The AEA and 2-AG treatment significantly attenuated the stess-induced decrease in DMI/mBW and EB (P < 0.05) and 2-AG treatment significantly attenuated the stess-induced decrease in ECM (P < 0.05). Graphs are presented as highest level of significant interaction, associated P-values can be found in Supplementary Table S3. Within-housing differences are indicated by * P < 0.05 and ** P < 0.01 (Tukey–Kramer).