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. 2021 Dec 8;11:23657. doi: 10.1038/s41598-021-02970-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Percent changes (%) in plasma NEFA (a), triglyceride (TG) (b) and cholesterol (c) concentrations after i.p. injections of NaCl (n = 7), AEA (n = 7) or 2-AG (n = 6) for 8 days relative to pre-treatment. Plasma NEFA concentatrions significantly decreased after 8 days of treatment with AEA compared to the control group (P < 0.001) and tended to increase to a lesser extend with 2-AG treatment compared to the control group (P = 0.05). No changes were detected for percent changes in plasma TG. Plasma cholesterol percent changes differed in the control group between diets, as indicated by the treatment x diet interaction (P < 0.05). Graphs are presented as highest level of significant interaction, associated P-values can be found in Supplementary Table S3. Treatment differences are indicated by # < 0.1, * P < 0.05 and *** P < 0.001 (Tukey–Kramer).