Table 1.
Relative mRNA abundances of hepatic genes involved in endocannabinoid and fat metabolism.
| Gene | NaCl | AEA | 2AG | P-value |
|---|---|---|---|---|
| Treatment | ||||
| ACAA2 | 0.99 ± 0.06 | 0.99 ± 0.06 | 1.01 ± 0.06 | 0.96 |
| ACACA | 0.82 ± 0.19 | 1.25 ± 0.19 | 1.14 ± 0.21 | 0.29 |
| CNR1 | 1.38 ± 0.22 | 0.96 ± 0.22 | 1.05 ± 0.24 | 0.39 |
| CPT1A | 1.09 ± 0.10 | 0.94 ± 0.10 | 1.04 ± 0.11 | 0.43 |
| DGAT1 | 1.13 ± 0.25 | 0.92 ± 0.25 | 1.38 ± 0.27 | 0.47 |
| DGAT2 | 1.21 ± 0.25 | 1.04 ± 0.25 | 1.19 ± 0.27 | 0.87 |
| FAAH | 1.13 ± 0.08 | 0.96 ± 0.08 | 0.97 ± 0.09 | 0.23 |
| GRP55 | 1.12 ± 0.19 | 0.97 ± 0.19 | 1.20 ± 0.20 | 0.70 |
| HADH | 1.04 ± 0.06 | 0.96 ± 0.06 | 1.03 ± 0.07 | 0.61 |
| PLAAT5 | 1.38 ± 0.42 | 1.31 ± 0.42 | 1.36 ± 0.45 | 0.99 |
| MGLL | 0.97 ± 0.16 | 1.16 ± 0.16 | 1.01 ± 0.17 | 0.65 |
| PPARA | 1.09 ± 0.19 | 1.32 ± 0.19 | 0.87 ± 0.21 | 0.31 |
| SREBF1 | 1.13 ± 0.11 | 1.11 ± 0.11 | 0.86 ± 0.12 | 0.22 |
Liver tissue samples were obtained from cows treated with NaCl (n = 7), AEA (n = 7) or 2AG (n = 6) for 9 days.
ACAA2, acetyl-COA acyltransferase 2; ACACA, acetyl-CoA carboxylase alpha; CNR1, cannabinoid receptor 1; CPT1A, carnitine palmitoyltransferase 1A; DGAT1/ 2, diacylglycerol O-acyltransferase 1/ 2; FAAH, fatty acid amide hydrolase; GPR55, G protein-coupled receptor 55; HADH, hydroxyacyl-CoA dehydrogenase; PLAAT5, phospholipase A and acyltransferase 5; MGLL, monoglyceride lipase; PPARA, peroxisome proliferator activated receptor alpha; SREBF1, sterol regulatory element binding transcription factor 1.