Table 2.
Summary of RAAS pathway genes, RT-qPCR primer sequence, and Ensembl ID used for the RT-qPCR analysis.
| Gene Name | Gene symbol | Function | Primer Sequences (5′- > 3′) | Transcript ID | Product length |
|---|---|---|---|---|---|
| Angiotensin II receptor, type 1a | agtr1a | G protein-coupled receptor for angiotensin II | F: CATCCGTGGGACCCATTTCA | ENSDART00000021528.7 | 154 |
| R: GCAGTAGCACGTGAGGATGA | |||||
| Angiotensin II receptor, type 1b | agtr1b | G protein-coupled receptor for angiotensin II | F: TTCATGCCGTTTGGCTCAGA | ENSDART00000066834.5 | 200 |
| R: GGTCCTCGCTCATTGCTGAT | |||||
| Angiotensin converting enzyme | ace | Metallopeptidase involved in the conversion of angiotensin I to angiotensin II | F: GAGCCAATCCTGGCTTCCAT | ENSDART00000114637.4 | 133 |
| R: CCGATGACGCTGAGAGTGAC | |||||
| Angiotensin converting enzyme 2 | ace2 | Transmembrane protein catalyzing angiotensin II hydrolysis. Main entry point for coronavirus | F: CTGATGCCTGTCTTCCAGCA | ENSDART00000003712.8 | 141 |
| R: TTTCATCCCAACCCTGCTCC | |||||
| Angiotensinogen | agt | Precursor molecule for angiotensin I and the substrate for renin produced in the liver | F: GGCTTCGACACCTCAAGGAA | ENSDART00000010918.5 | 192 |
| R:ACACCACCTTGTTGAGTACCTTA | |||||
| Angiotensin II receptor, type 2 | at2 | G protein-coupled receptor. Regulation of aldosterone secretion | F: GTTCACGAACATCAGAACTCCC | ENSDART00000051532.5 | 188 |
| R: TGAGGCTGTAAAAGGCAGGG | |||||
| Elongation factor 1 alpha 1 | elf1a1 | Housekeeping gene for RT-qCPR | F: CTTCTCAGGCTGACTGTGC | Adopted from McCurley et al29 | – |
| R: CCGCTAGCATTACCCTCC |