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. 2021 Dec 8;12:7130. doi: 10.1038/s41467-021-27460-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Heat map of fatty-acid transporter genes differentially expressed by HSCs from control, S. typhimurium (Sal), and LPS-treated animals. b Flow cytometry analysis of CD36 expression in the HSC of S. typhimurium and LPS-treated animals. n = 5 in each group. c C57BL/6 J mice were pre-treated with SSO for one hour before treatment with LPS for 16 hours. n = 5 in each group. d The cells from control, LPS, or LPS and SSO-treated animals were isolated and long-chain fatty-acid (LCFA) uptake was measured using the QBT assay. n = 5 in each group. e Percentage of cycling HSCs as measured by Ki67-positive cells after pre-treatment followed by LPS treatment. n = 5. f Schematic diagram of the experiment. g The LK cells from LPS-treated CD36^−/− or WT (CD36^+/+) mice were isolated and long-chain fatty-acid (LCFA) uptake was measured using the QBT assay. n = 5. h Percentage of cycling HSCs from LPS-treated CD36^−/− or WT (CD36^+/+) mice as measured by Ki67-positive. n = 5 mice in each group. i CD36^−/− or WT (CD36^+/+) were infected with S. typhimurium (Sal). Representative live-cell fluorescent microscopy images of LSK cells isolated from the mice, Sca1 membrane stain (red), Bodipy 493/503 (green) and Hoechst 33342 (blue), 20 LSK cells from five mice in each condition. j CD36^+/+ and CD36^−/− mice were treated with S. typhimurium the animals were killed and the LSK population was isolated by FACS, OCR was measured. n = 5. k Basal ECAR compared to basal OCR levels before and after treatment with LPS or S. typhimurium. Basal OCR normalized to rotenone. n = 5. l Schematic diagram of experimental design. m Mice were injected LPS for 16 hours then treated with FFA-SS-luc and imaged using bioluminescence (FFA-luciferin+LPS). Densitometry of the bioluminescent images to determine fluorescence intensity. n = 4 for each group. The Mann–Whitney U test (two-tailed) was used and the Kruskal–Wallis test followed by Dunn’s multiple comparison post hoc test was used *P < 0.05 **p < 0. 01. Source data are provided as a Source Data file.